I think you're referring to the PEP8 max line length visual? The one which shows you if you are crossing the recommended max line length?
If so, this should be configured in your .vimrc with a line like:
let g:pymode_options_max_line_length = 119
where 119 would be the last character position before the vertical line.

If you want to completely remove it, this worked for me:
let g:pymode_options_colorcolumn = 0

@qazip does that works for you? :)

Thanks @jakecolston for answering!

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