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| 1 | +Reading and Writing of Basic sequence file formats |
| 2 | +================================================== |
| 3 | + |
| 4 | + |
| 5 | +TODO: needs more examples |
| 6 | + |
| 7 | + |
| 8 | +## FASTA |
| 9 | + |
| 10 | +BioJava can be used to parse large FASTA files. The example below can parse a 1GB (compressed) version of TREMBL with standard memory settings. |
| 11 | + |
| 12 | + |
| 13 | +```java |
| 14 | + |
| 15 | + |
| 16 | + |
| 17 | + /** Download a large file, e.g. ftp://ftp.uniprot.org/pub/databases/uniprot/current_release/knowledgebase/complete/uniprot_trembl.fasta.gz |
| 18 | + * and pass in path to local location of file |
| 19 | + * |
| 20 | + * @param args |
| 21 | + */ |
| 22 | + public static void main(String[] args) { |
| 23 | + |
| 24 | + if ( args.length < 1) { |
| 25 | + System.err.println("First argument needs to be path to fasta file"); |
| 26 | + return; |
| 27 | + } |
| 28 | + |
| 29 | + File f = new File(args[0]); |
| 30 | + |
| 31 | + if ( ! f.exists()) { |
| 32 | + System.err.println("File does not exist " + args[0]); |
| 33 | + return; |
| 34 | + } |
| 35 | + |
| 36 | + try { |
| 37 | + |
| 38 | + // automatically uncompresses files using InputStreamProvider |
| 39 | + InputStreamProvider isp = new InputStreamProvider(); |
| 40 | + |
| 41 | + InputStream inStream = isp.getInputStream(f); |
| 42 | + |
| 43 | + FastaReader<ProteinSequence, AminoAcidCompound> fastaReader = new FastaReader<ProteinSequence, AminoAcidCompound>( |
| 44 | + inStream, |
| 45 | + new GenericFastaHeaderParser<ProteinSequence, AminoAcidCompound>(), |
| 46 | + new ProteinSequenceCreator(AminoAcidCompoundSet.getAminoAcidCompoundSet())); |
| 47 | + |
| 48 | + LinkedHashMap<String, ProteinSequence> b; |
| 49 | + |
| 50 | + |
| 51 | + int nrSeq = 0; |
| 52 | + |
| 53 | + while ((b = fastaReader.process(10)) != null) { |
| 54 | + for (String key : b.keySet()) { |
| 55 | + nrSeq++; |
| 56 | + System.out.println(nrSeq + " : " + key + " " + b.get(key)); |
| 57 | + } |
| 58 | + |
| 59 | + } |
| 60 | + } catch (Exception ex) { |
| 61 | + Logger.getLogger(ParseFastaFileDemo.class.getName()).log(Level.SEVERE, null, ex); |
| 62 | + } |
| 63 | + } |
| 64 | +``` |
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