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illumination patterns: 1: Top half circle 2: Bottom 3: Left 4: Right Exprimental parameters: imaging: 0.4 NA magnification 40x (20x objective and an extra 2x at the front port of TE300) camera: pixel size 6.5um LEDs: central wavelength: 530nm LED spacing: 4mm distance from array to sample: 69.5mm illumination NA: ~0.4